Computational protocol: Low level of Lck kinase in Th2 cells limits expression of CD4 co-receptor and S73 phosphorylation of transcription factor c-Jun

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Protocol publication

[…] Flow cytometry analysis was performed using BD LSRFortessa and BD Canto analyzers (Becton Dickinson, Franklin Lakes, NJ) and antibodies listed in Supplementary Table . An average of 20,000 cells was used per sample. Data collection was performed by BD FACSDiva software. FlowJo software was used for data analysis. Cell sorting was performed using BD Influx sorter operated under BD FACSTM software. An average of 106 GFP+ cells was sorted per sample. Propidium Iodide and Fixable Viability Dye eFluor® 780 (eBioscience, San Diego, CA) were used for exclusion of dead cells. […]

Pipeline specifications

Software tools BD FACSDiva, FlowJo
Application Flow cytometry