Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Oct 23 2012
Last update date: Nov 6 2013
Access: Public
Chemicals: Estradiol
Dataset link To elucidate the role of estradiol- 17β (E2) in the regulation of corpus luteum function

Experimental Protocol


The objective of this study was to identify the effect of E2 deprivation and supplementation on CL function and temporal changes in the transcriptome of pregnant rat CL during day 12 to 16 of pregnancy following E2 deprivation by Anastrozole (AI, Aromatase inhibitor) treatment in pregnant rats, AI (1mg/ kg bw orally) treatment beginning on day 12 daily for 4 days. In the E2 supplementation experiments, together with AI, the rats were treated with E2 (10µg/ day s.c.). CL tissues were collected on day 12 (no treatment; n=3 animals/ time point), day 16 (vehicle treatment; n=3 animals/ time point), day 16 (AI treatment; n=3 animals/ time point) and day 16 (AI+ E2 treatment; n=3 animals/ time point) to examine gene expression changes associated with E2 deprivation and recovery in the system. Following retrieval of CL from the ovary, corpora lutea were flash frozen in liquid nitrogen and stored at -70oC for the purpose of RNA isolation. The isolated RNA was labelled and hybridized to Affymetrix GeneChip® Rat Gene 1.0 ST arrays as per the manufacturer’s instructions.

Repositories


GEO

GSE41735

ArrayExpress

E-GEOD-41735

BioProject

PRJNA178091

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Contact


SUDESHNA TRIPATHY
S Tripathy