Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 4
Release date: Jun 23 2016
Last update date: Jun 20 2018
Access: Public
Chemicals: Carnitine, Fatty Acids, Glucose, Hydrocortisone
Dataset link Integrated metabolomic and transcriptome analyses reveal finishing forage affects metabolic pathways related to beef quality and animal welfare

Experimental Protocol


The steers came from a closed Wye Angus herd with very similar genetics. The grass-fed group was comprised of steers that received alfalfa and orchard grass hay, clover and orchard grass pasture, or orchard grass and alfalfa pasture. The grass-fed individuals consumed grazed alfalfa upon availability and bales during winter and were not exposed to any corn, any form of grain or feed by-products. The alfalfa and grass hay were harvested from land that has had minimal fertilizer and no application of pesticides or inorganic chemicals. The control group was fed a conventional diet consisting of corn silage, soybean, shelled corn and minerals. The pastures were managed as organic lands–without fertilizers, pesticides or any chemical additives. At the slaughter plant, 10 ml whole blood sample from the jugular vein was collected in EDTA tubes and directly storage at -80°C. Then, a small piece of longissimus dorsi muscle was obtained from each hot carcass at the level of the 12th intercostal space and immediately frozen in dry ice for posterior analysis.

Repositories


GEO

GSE70248

ArrayExpress

E-GEOD-70248

ENA

SRP059849

BioProject

PRJNA288034

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Contact


Jose Carrillo
Jose Adrian Carrillo

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