|Number of samples:||24|
|Release date:||Apr 24 2014|
|Last update date:||Oct 18 2018|
|Dataset link||Human intestinal tissue with adult stem cell properties derived from pluripotent stem cells|
RNA from primary organoid samples was isolated from organoid lines that were both cultured for 1-6 months and derived from duodenum, ileum, or rectum biopsies of human subjects as described previously (Sato et al., Gastroenterology 2011) grown in media called WENR+inhibitors. RNA was also isolated from various steps in the culturing and differentiation protocol.