Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Dec 26 2005
Last update date: Mar 16 2012
Access: Public
Diseases: Myopathies, Structural, Congenital
Dataset link Differential expression between mature zone of the olfactory organ and the dactyl of the walking legs, a taste organ

Experimental Protocol


We designed a cDNA microarray that consisted of five subarrays to cover all of the cDNA species in our library. We generated two of the sub-arrays, each with 1,152 clones spotted in duplicate, giving 2,304 spots per sub-array. A common set of 96 clones, consisting of positive and negative control sequences, was spotted on each sub-array. Each cloned cDNA insert was amplified by PCR and spotted on nylon membranes using an Affymetrix 417 Arrayer according to protocols supplied by Dr. Kevin Becker (Tanaka et al., 2000; Barrett et al., 2001). Arrays were hybridized against 33P-labeled cDNA produced by oligo-dT priming of reverse transcription using the RadPrime DNA labeling kit (Invitrogen). Hybridization was done in quadruplicate at 50˚C in 50 ml tubes in a hybridization oven as described in Barrett et al. (2001).

Repositories


GEO

GSE3785

BioProject

PRJNA93979

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Contact


Timothy McClintock