Computational protocol: Suppression of Nrf2 Activity by Chestnut Leaf Extract Increases Chemosensitivity of Breast Cancer Stem Cells to Paclitaxel

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Protocol publication

[…] Cells were cultured in a 6-well plate coated with Matrigel (2.5% v/v in PBS; Corning, Corning, NY, USA) in the absence or presence of the test sample or paclitaxel for 5 days. Colonies formed from the plated cells in each well were optically visualized via crystal violet staining. The scanned images of each well of the 6-well plate were analyzed using ImageJ software, 1.48v (developed at the National Institutes of Health). [...] The obtained data were analyzed via one-way analysis of variance and Duncan's multiple range test using the SPSS statistics 22 software (SPSS Inc., Chicago, IL, USA). The p-values less than 0.05 were considered significant. Statistical differences were indicated using asterisks, hashtags, or different alphabetical letters. […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Diseases Neoplasms
Chemicals Oxygen, Paclitaxel