Similar protocols

Protocol publication

[…] In order to assess the integrity of the microbial cells, biofilms were developed in a Lab-Tek TM coverslip chambers (Nunc) as described above, and treated with standard drugs and EOs at 1 mg/mL. The samples were washed twice with 3% glutaraldehyde in phosphate buffer (pH 7.4) and fixed in glutaraldehyde 0.15 M 2.5% (v/v) at room temperature for 12 h. The dehydrated cells were submitted to sequential baths of ethanol at concentrations of 50%, 70%, 90% and absolute ethanol twice, until the dried at the critical point, then coated with gold in a Metalizer and observed using a Scanning Electron Microscope (Jeol model JSM 5600 Lv) []. [...] Statistical analysis was performed with one-way ANOVA and p-values ≤0.05 considered statistically significant. The inhibition of biofilm formation data were compared by Tukey test. The statistical test was run using STATISTICA® v.8.0 (Stafsoft, USA) system software. […]

Pipeline specifications

Software tools ProteinsPlus, Statistica
Applications Miscellaneous, cryo-EM
Organisms Candida albicans, Streptococcus sanguinis, Fusobacterium nucleatum, Porphyromonas gingivalis, Streptococcus mitis, Coriandrum sativum
Chemicals Alcohols, Aldehydes, Sesquiterpenes, Terpenes, Monoterpenes