Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jan 12 2016
Last update date: Aug 23 2018
Access: Public
Diseases: Skin Diseases
Dataset link Beta-catenin stabilization of skin fibroblasts causes fibrotic lesions by preventing adipocyte differentiation of the reticular dermis

Experimental Protocol


The dermis was separated from back skin of PDGFRAeGFP postnatal pups (P2) by incubation with thermolysin (0.25 mg/ml) (Sigma T7902) overnight at 4° and further processed as previously described (Collins et al., 2011). Cells were labeled in PBS + 10% FBS TruStain fcX anti-mouse blocking buffer with the following antibodies: anti-mouse Ly-6A/E (Sca-1)-Alexa Fluor-700 17. Two populations of cells were collected in triplicate. The PDGFRaH2BeGFP/Sca1- and the PDGFRaH2BeGFP/Sca1+. RNA was isolated and prepared for microarray analysis and hybridized to Affymetrix MG430.2A arrays. C, digested in DMEM + 10% FBS containing 2.5 mg/mL collagenase I (Gibco 17100- 017), and further processed

Repositories


GEO

GSE76751

ArrayExpress

E-GEOD-76751

BioProject

PRJNA308498

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Contact


Ryan Driskell
Ryan R Driskell

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