Computational protocol: V-ATPase Proton Pumping Activity Is Required for Adult Zebrafish Appendage Regeneration

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Protocol publication

[…] For in situ hybridization and in vivo protocols, images were obtained with a Leica Z6APO stereomicroscope equipped with a Leica DFC320 color camera. Immunostainings were imaged on a Zeiss LSM 710 confocal microscope. The ImageJ software was used to analyze the Z stacks and to measure regenerate area and blastema length. All statistical analyses were done on SPSS 16.0 software. […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Laser scanning microscopy, Microscopic phenotype analysis
Organisms Danio rerio