|Number of samples:||17|
|Release date:||Jul 10 2017|
|Last update date:||Oct 24 2018|
|Dataset link||DNA methylation drift as a marker of lifespan in mammals [mouse]|
Digital restriction enzyme analysis of methylation (DREAM) was performed to determine the methylation profile of mouse whole blood genomic DNA. Genomic DNA spiked in with unmethylated, partially methylated and fully methylated standards was sequentially cut at CCCGGG sites with the methylation-sensitive enzyme SmaI (blunt ends) and its methylation-tolerant neoschizomer XmaI (5'CCGG overhangs), creating different end sequences that represented methylation status of the CCCGGG sites. These end sequences were analyzed by Illumina high throughput sequencing. Methylation status at individual CCCGGG sites across the genome was determined by counting the methylated reads with the CCGGG signature and unmethylated reads with the GGG signature at the beginnings of the sequencing reads after alignment to the mouse genome.
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