Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 17
Release date: Jul 10 2017
Last update date: Oct 24 2018
Access: Public
Chemicals: 5-Methylcytosine
Dataset link DNA methylation drift as a marker of lifespan in mammals [mouse]

Experimental Protocol


Digital restriction enzyme analysis of methylation (DREAM) was performed to determine the methylation profile of mouse whole blood genomic DNA. Genomic DNA spiked in with unmethylated, partially methylated and fully methylated standards was sequentially cut at CCCGGG sites with the methylation-sensitive enzyme SmaI (blunt ends) and its methylation-tolerant neoschizomer XmaI (5'CCGG overhangs), creating different end sequences that represented methylation status of the CCCGGG sites. These end sequences were analyzed by Illumina high throughput sequencing. Methylation status at individual CCCGGG sites across the genome was determined by counting the methylated reads with the CCGGG signature and unmethylated reads with the GGG signature at the beginnings of the sequencing reads after alignment to the mouse genome.

Repositories


GEO

GSE75495

ENA

SRP066799

BioProject

PRJNA304429

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Contact


Jaroslav Jelinek

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