Computational protocol: Postembryonic development of transit amplifying neuroblast lineages in the Drosophila brain

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Protocol publication

[…] Fluorescently stained nervous systems were imaged using a Leica TCS SP scanning confocal microscope using 40× and 63× oil-immersion objectives. Z stacks were collected with optical sections at 1-μm intervals. Scanned pictures were visualized in ImageJ [] and analyzed. For the illustration of findings the most representative scans were chosen and processed (not related non-DM lineages were excluded using the 'lasso' tool). Pictures are presented as 'thick-section' merges projected as a flat image using ImageJ []. Figures were assembled using Adobe Illustrator and Photoshop. […]

Pipeline specifications

Software tools ImageJ, Adobe Illustrator
Applications Miscellaneous, Laser scanning microscopy, Microscopic phenotype analysis
Organisms Drosophila melanogaster