Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 14
Release date: Jan 7 2014
Last update date: Aug 23 2018
Access: Public
Genes: CCR4, Gsk3b, CNOT2, CNOT1, CNOT3, TRIM28
Taxon: Mus musculus
Dataset link Identification of Ccr4-Not complex as a regulator of transition from partial to genuine iPS cells

Experimental Protocol


Two partial iPSC clones (2B1 and 5C5) cultured under conventional culture condition with leukemia inhibitory factor (LIF) and serum and those converted to iPSCs by the exposure to 2i condition were used for RNA source. Partial iPSCs (2B1) cultured under conventional condition in which either one of Cnot1, Cnot2, Cnot3 and Trim28 cDNA or these factors were combinatorially incorporated after retrovirus infection and those in which empty vector or Nanog were stably integrated were also used for RNA source. In addition, embryonic fibroblasts and embryonic stem cells (ESCs) from 13.5 dpc embryos and blastocysts, respectively, from Nanog-GFP transgenic mice and wild-type ESC line (EBRTcH3) were used for RNA source.

Repositories


GEO

GSE44339

ArrayExpress

E-GEOD-44339

BioProject

PRJNA189503

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Contact


Akihiko Okuda