|Application:||Gene expression microarray analysis|
|Number of samples:||14|
|Release date:||Jan 7 2014|
|Last update date:||Aug 23 2018|
|Genes:||CCR4, Gsk3b, CNOT2, CNOT1, CNOT3, TRIM28|
|Dataset link||Identification of Ccr4-Not complex as a regulator of transition from partial to genuine iPS cells|
Two partial iPSC clones (2B1 and 5C5) cultured under conventional culture condition with leukemia inhibitory factor (LIF) and serum and those converted to iPSCs by the exposure to 2i condition were used for RNA source. Partial iPSCs (2B1) cultured under conventional condition in which either one of Cnot1, Cnot2, Cnot3 and Trim28 cDNA or these factors were combinatorially incorporated after retrovirus infection and those in which empty vector or Nanog were stably integrated were also used for RNA source. In addition, embryonic fibroblasts and embryonic stem cells (ESCs) from 13.5 dpc embryos and blastocysts, respectively, from Nanog-GFP transgenic mice and wild-type ESC line (EBRTcH3) were used for RNA source.