Dataset features


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Apr 3 2018
Last update date: Apr 4 2018
Access: Public
Diseases: Skin Diseases
Dataset link Comparative expression data between the epidermis and dermis of wild-type (Pparb/d^fl/fl) and fibroblast-selective knockout Pparb/d (FSPCre-Pparb/d^fl/fl) mice

Experimental Protocol

Pparb/d^fl/fl mice, having the exon 4 of their Pparb/d gene flanked by loxP sites, were mated with mice harbouring the Cre transgene under the control of the FSP promoter. FSPCre-Pparb/d^fl/+ progenies were then bred with Pparb/d^fl/fl mice to obtain FSPCre-Pparb/d^fl/fl mice and the wild-type control mice. To sustain this genotype, we bred FSPCre-Pparb/d^fl/fl progenies with Pparb/d^fl/fl mice. Mice were housed in a specific-pathogen free facility with a 12 h/12 h light/dark cycle. Food and water were provided ad libitum. Animal experiments were carried out in accordance to the guidelines of the University Institutional Animal Care and Use Committee (IACUC, ARF-SBS/NIE-A0216AZ), Singapore. Mice in the telogen phase of their hair cycle were anaesthetized and depilated on their dorsal back before being subjected to a biopsy punch (~4 mm). The skin biopsy was then treated with 3.8% ammonium thiocyanate (Sigma, USA) in 1X PBS for 30 min at 37 oC. Subsequently, the epidermis was mechanically separated from the dermis with a pair of forceps. The dermis was then curetted with a 30-gauge needle. RNA was extracted from the epidermis and dermis with Trizol according to the manufacturer’s protocol. Further sample processing of the RNA was carried out using Applause® WT-Amp ST System (NuGEN), and microarray experiments were performed on GeneChip® Mouse Gene 1.0 ST arrays according to the manufacturer’s instructions.








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