|Application:||miRNA array analysis|
|Number of samples:||6|
|Release date:||Sep 12 2018|
|Last update date:||Sep 13 2018|
|Diseases:||Leukemia, Leukemia, Hairy Cell, Neoplasms, Leukemia, Myelogenous, Chronic, BCR-ABL Positive|
|Taxon:||Homo sapiens, Mus musculus|
|Dataset link||Identification of a novel lncRNA family that is required for efficient cellular transformation by Bcr-Abl oncogene|
Total RNAs were isolated from three independent groups of K562 cells treated with Imatinib or DMSO respectively. Then, total RNA was quantified by the NanoDrop ND-2000 (Thermo Scientific) and the RNA integrity was assessed using Agilent Bioanalyzer 2100 (Agilent Technologies).The sample labeling, microarray hybridization and washing were performed based on the manufacturerâs standard protocols. Briefly, total RNA were transcribed to double strand cDNA, then synthesized into cRNA and labeled with Cyanine-3-CTP. The labeled cRNAs were hybridized onto the microarray. After washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies).