Dataset features

Specifications


Application: miRNA array analysis
Number of samples: 6
Release date: Sep 12 2018
Last update date: Sep 13 2018
Access: Public
Diseases: Leukemia, Leukemia, Hairy Cell, Neoplasms, Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Chemicals: Tyrosine
Genes: ABL1, UBP1
Taxon: Homo sapiens, Mus musculus
Dataset link Identification of a novel lncRNA family that is required for efficient cellular transformation by Bcr-Abl oncogene

Experimental Protocol


Total RNAs were isolated from three independent groups of K562 cells treated with Imatinib or DMSO respectively. Then, total RNA was quantified by the NanoDrop ND-2000 (Thermo Scientific) and the RNA integrity was assessed using Agilent Bioanalyzer 2100 (Agilent Technologies).The sample labeling, microarray hybridization and washing were performed based on the manufacturer’s standard protocols. Briefly, total RNA were transcribed to double strand cDNA, then synthesized into cRNA and labeled with Cyanine-3-CTP. The labeled cRNAs were hybridized onto the microarray. After washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies).

Repositories


GEO

GSE119770

BioProject

PRJNA490264

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Contact


Xuefei Wang