Computational protocol: Wheat Transcription Factor TaAREB3 Participates in Drought and Freezing Tolerances in Arabidopsis

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Protocol publication

[…] Using sequences of Arabidopsis AREB family members as references, a complete wheat AREB cDNA (named as TaAREB3) sequence information was obtained from the wheat A and D genome databases by tBLASTn , . The full-length cDNA of the TaAREB3 gene was then cloned from wheat total RNA by reverse transcription polymerase chain reaction (RT-PCR). Through amino acid sequence analyses using protein BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi) various bZIP genes were obtained from plant species, such as Arabidopsis, rice, barley, and Brachypodium distachyon. Phylogenetic analysis was performed by the maximum likelihood method using Molecular Evolutionary Genetics Analysis (MEGA) 5 software. […]

Pipeline specifications

Software tools TBLASTN, DELTA-BLAST, MEGA
Databases Full Length cDNA
Applications Phylogenetics, Amino acid sequence alignment
Organisms Arabidopsis thaliana, Triticum aestivum, Nicotiana tabacum
Chemicals Abscisic Acid