Similar protocols

Protocol publication

[…] res for bacteria and yeasts were performed using standard microbiological methods. For all samples, macroscopically distinct colonies were isolated from pure culture, and standard methods for identification, typing and establishing antibiotic sensitivity patterns were used., Sequence data for each clinical strain were formatted and assembled by the Seqman program of DNASTAR Software (v5.0). The sequences of EV-D68 strains that were newly obtained in Chongqing have been deposited in GenBank under the accession numbers KT803582–KT803606. To study the distribution and diversity of EV-D68 identified in this study compared to other EV-D68 strains, multiple sequence alignments were made using the ClustalW function of the BioEdit program (v7.0.9). Phylogenetic trees were constructed using the MEGA neighbor-joining algorithm (5.05). The statistical significances of the tree topologies were tested via bootstrapping (1000 replicates). Only bootstrap values >70% are shown in each tree. A phylogenetic tree that was constructed with 369 bp of VP1 gene nucleotide sequences showed that modern EV-D68 strains were divided into three primary clades (A, B and C) according to the classification previously described by Tokarz (). Nearly full-length nucleotide sequences that correspond to nt 145–7203 of the EV-D68 prototype strain (GenBank accession no. AY426531) were obtained from 13 strains of EV-D68 in the current study. A phylogenetic tree was constructed based on this region of nucleoti […]

Pipeline specifications

Software tools Clustal W, BioEdit, MEGA
Organisms Homo sapiens
Diseases Asthma, Infection, Respiratory Sounds, Respiratory Tract Infections, Sprains and Strains, Wiskott-Aldrich Syndrome, Precursor Cell Lymphoblastic Leukemia-Lymphoma