|Application:||Gene expression microarray analysis|
|Number of samples:||14|
|Release date:||Jan 11 2017|
|Last update date:||Aug 23 2018|
|Diseases:||Deficiency Diseases, Leukemia, Lymphoid, Lymphoma, Follicular, Neoplasms, Lymphoma, Large B-Cell, Diffuse|
|Dataset link||Gene expression profile analysis of germinal center B cells from conditional Crebbp knockout mice and littermate controls|
Murine germinal center B cells (B220+CD95+PNA+) were sorted from the spleen of conditional Crebbp knock-out, Crebbp heterozygous and Crebbp wild type mice (crossed with the Cg1-Cre deletor strain) and sacrificed at day 12 after immunization with sheep red blood cells (SRBC)(n=4WT, 5HET and 5KO). Total RNA was extracted from single cell suspensions and processed according to the Ovation RNA Amplification System and Encore® Biotin Module protocols (both from NuGEN). Samples were analyzed on Affymetrix Mouse Genome 430 2.0 arrays.