Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 14
Release date: Jan 11 2017
Last update date: Aug 23 2018
Access: Public
Diseases: Deficiency Diseases, Leukemia, Lymphoid, Lymphoma, Follicular, Neoplasms, Lymphoma, Large B-Cell, Diffuse
Dataset link Gene expression profile analysis of germinal center B cells from conditional Crebbp knockout mice and littermate controls

Experimental Protocol


Murine germinal center B cells (B220+CD95+PNA+) were sorted from the spleen of conditional Crebbp knock-out, Crebbp heterozygous and Crebbp wild type mice (crossed with the Cg1-Cre deletor strain) and sacrificed at day 12 after immunization with sheep red blood cells (SRBC)(n=4WT, 5HET and 5KO). Total RNA was extracted from single cell suspensions and processed according to the Ovation RNA Amplification System and Encore® Biotin Module protocols (both from NuGEN). Samples were analyzed on Affymetrix Mouse Genome 430 2.0 arrays.

Repositories


GEO

GSE88799

BioProject

PRJNA348677

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Contact


Laura Pasqualucci