Computational protocol: Cloning and purification of the first termicin-like peptide from the cockroach Eupolyphaga sinensis

Similar protocols

Protocol publication

[…] The signal peptide was predicted with the SignalP 4.1 program ( The signal peptide cleavage site was confirmed by the determined sequence of mature Es-termicin. The amino acid sequence of mature polypeptide was compared against public databases ( The resulting sequences were aligned using the ClustalW2 ( The alignment was imported into MEGA software to construct phylogenetic tree by the maximum likelihood method []. […]

Pipeline specifications

Software tools SignalP, Clustal W, MEGA
Application Phylogenetics
Organisms Eupolyphaga sinensis, Candida albicans, Enterococcus faecalis
Diseases Mycoses
Chemicals Amino Acids