Computational protocol: Cloning and purification of the first termicin-like peptide from the cockroach Eupolyphaga sinensis

Similar protocols

Protocol publication

[…] The signal peptide was predicted with the SignalP 4.1 program (http://www.cbs.dtu.dk/services/SignalP/). The signal peptide cleavage site was confirmed by the determined sequence of mature Es-termicin. The amino acid sequence of mature polypeptide was compared against public databases (http://blast.ncbi.nlm.nih.gov/Blast.cgi). The resulting sequences were aligned using the ClustalW2 (http://www.ebi.ac.uk/Tools/clustalw2/index.html). The alignment was imported into MEGA software to construct phylogenetic tree by the maximum likelihood method []. […]

Pipeline specifications

Software tools SignalP, Clustal W, MEGA
Application Phylogenetics
Organisms Eupolyphaga sinensis, Candida albicans, Enterococcus faecalis
Diseases Mycoses
Chemicals Amino Acids