|Application:||Gene expression microarray analysis|
|Number of samples:||27|
|Release date:||Sep 23 2008|
|Last update date:||Mar 20 2012|
|Diseases:||Intervertebral Disc Degeneration|
|Dataset link||Eye imaginal disc development|
This study took advantage of the spatial arrangement of undifferentiated and differentiating cells on either side of the MF in Drosophila melanogaster eye imaginal discs. To identify genes that are important to the undifferentiated state of proliferating eye disc cells, or to cells exiting the mitotic program to differentiate, RNA was isolated from posterior cells and from whole eye discs. Disc fragments posterior to the MF were obtained by micro-dissection from the w1118 strain that differentiates eyes normally (except for pigmentation). Whole eye discs were from various mutants that either increase the proportion of differentiating photoreceptor cells (extra R8 phenotype: rough X63 (roX63), and Su(roDom)519 (Chanut et al., 2000) or reduce the proportion of photoreceptor cells due to a stop furrow phenotype (atonal1 (ato1), hedgehog1 (hh1), roughDominant (roDom), and Enhancer(roDom)2033 (E(roDom)2033) (Dokucu et al., 1996; Chanut et al., 2000). After purification, the RNA populations were processed by linear amplification and hybridized to custom-made DNA glass microarrays. Hybridization experiments were performed comparing posterior fragments or discs with abnormal numbers of photoreceptors to “normal” discs; a minimum of two independent replicates were made for each of nine different experimental conditions.