|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Jan 1 2013|
|Last update date:||Apr 2 2013|
|Diseases:||Deficiency Diseases, Metabolic Diseases, Neoplasms|
|Chemicals:||Glucose, Glutamic Acid|
|Dataset link||PKCz restrains cancer cell transformation by inhibiting nutrient stress-induced metabolic reprogramming|
SW480 cells were infected with lentivirus expressing PKCz RNA interference (shPKCz) and a control lentivirus (shNT). The elimination of endogenous PKCz with shPKCz transfection was >90% efficient. Cells transfected with shNT had unaltered levels of PKCz. To mimic the conditions of nutrient stress to which tumor cells are normally exposed, both cell types were initially seeded in DMEM with 10% FBS, and cultured without subsequent further media changes, which led to nutrient exhaustion over time. Total RNA was harvested from three independent biological replicates for shPKCz and shNT transfected cells.