Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Jan 1 2013
Last update date: Apr 2 2013
Access: Public
Diseases: Deficiency Diseases, Metabolic Diseases, Neoplasms
Chemicals: Glucose, Glutamic Acid
Dataset link PKCz restrains cancer cell transformation by inhibiting nutrient stress-induced metabolic reprogramming

Experimental Protocol


SW480 cells were infected with lentivirus expressing PKCz RNA interference (shPKCz) and a control lentivirus (shNT). The elimination of endogenous PKCz with shPKCz transfection was >90% efficient. Cells transfected with shNT had unaltered levels of PKCz. To mimic the conditions of nutrient stress to which tumor cells are normally exposed, both cell types were initially seeded in DMEM with 10% FBS, and cultured without subsequent further media changes, which led to nutrient exhaustion over time. Total RNA was harvested from three independent biological replicates for shPKCz and shNT transfected cells.

Repositories


GEO

GSE42186

ArrayExpress

E-GEOD-42186

BioProject

PRJNA179262

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