Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Dec 2 2015
Last update date: Jan 18 2016
Access: Public
Diseases: Cystic Fibrosis, Artificial Lens Implant Migration
Chemicals: Calcium, Pyocyanine, Tobramycin
Dataset link Response of Pseudomonas strains PAO1 and FRD1 to 10 mM added calcium in planktonic and biofilm conditions.

Experimental Protocol


Two-condition experiment: 0 mM calcium vs. 10 mM calcium. For each of the four biological sample types, cells were grown with and without 10 mM added calcium, and co-hybridized to two color arrays. Dye swaps were performed on an additional array. Two independent biological replicates, grown on different days, were also co-hybridized on two additional arrays (dye swap). In this manner, four biological replicates were co-hybridized to four arrays, for four conditions, resulting in 16 two-color arrays deposited. Each array contained one biological growth condition at 0 at 10 mM calcium.

Repositories


GEO

GSE74445

BioProject

PRJNA300443

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Contact


Kerry Williamson