Computational protocol: The Stress Response Regulator AflSkn7 Influences Morphological Development, Stress Response, and Pathogenicity in the Fungus Aspergillus flavus

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Protocol publication

[…] The AflSkn7 (AFL2G_02624) sequence was retrieved from the Broad Institute database [] Sequences of other Skn7 orthologs were downloaded from the National Center for Biotechnology Information database using the BLASTP algorithm with the AflSkn7 protein sequence as a query. Domains were predicted using the Simple Modular Architecture Research Tool [] and drawn with the Illustrator for Biological Sequences program []. Sequences were aligned using ClustalW, and a phylogenetic tree was generated with MEGA5.1 (Center for Evolutionary Medicine and Informatics, Tempe, AZ, USA, 2012). […]

Pipeline specifications

Software tools BLASTP, Clustal W, MEGA
Databases SMART
Application Phylogenetics
Organisms Aspergillus flavus
Chemicals Aflatoxins