Dataset features


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Mar 1 2006
Last update date: Mar 16 2012
Access: Public
Diseases: Colonic Neoplasms, Neoplasms, Colorectal Neoplasms, Tumor Lysis Syndrome
Dataset link Comparative study of gene expression in human colorectal cancer tissues and normal mucosa

Experimental Protocol

We used cDNA microarrays to compare gene expression profiles of colorectal biopsies from 25 CRC patients and 13 normal mucosa from adjacent noncancerous tissues. Normal samples were pooled in equimolar amounts to generate a single standard reference RNA employed as a control in each microarray hybridisation. For half of microarray hybridisations, total RNA isolated from each tumour sample were retrotranscribed and labelled with Cy5-dCTP and cDNA obtained from the standard reference RNA was labelled with Cy3-dCTP; the fluorochromes were inverted in the second half of microarrays with the purpose of reducing dye bias. Findings were validated by real time PCR; in addition, western blotting and immunochemistry analysis were carried out as further confirmation of differential expression at a protein level.








Michele Bianchini