Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 65
Release date: Dec 1 2008
Last update date: Oct 29 2018
Access: Public
Diseases: Breast Neoplasms, Neoplasms
Chemicals: Estrogens, Tamoxifen, Tyrosine
Dataset link Expression data from MCF7 wt and MCF7/HER2-18 xenografts

Experimental Protocol


MCF7 xenografts were established in ovariectomized five to six week-old nu/nu athymic nude mice supplemented with 0.25 mg 21 day release estrogen pellets by inoculating subcutaneously (s.c.) 5E-6 cells. When tumors reached the size of 150-200 mm3 (3-5 weeks), the animals were randomly allocated to continued estrogen (E2), continued estrogen with gefitinib (E2+G; 100mg/kg, 5 days/week), estrogen withdrawal alone (ED; by removal of the estrogen pellets), and estrogen withdrawal plus tamoxifen citrate (Tam; 500 microg/animal s.c. in peanut oil, 5 days/week), with either gefitinib (Tam+G; 100mg/kg, 5 days/week) or vehicle (1% Tween 80) administered via gavage, as well as estrogen withdrawal plus fulvestrant (ICI 182,780) in the MCF7 wt model (Fulv; 5mg/mouse s.c. once weekly), and estrogen withdrawal with gefitinib (ED+G). Tumors were harvested for molecular studies when they became resistant to treatment and reached the size of 1000 mm3 (n=7). Refer to individual Series

Repositories


GEO

GSE8141

ArrayExpress

E-GEOD-8141

BioProject

PRJNA101027

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Contact


Chad Creighton