Computational protocol: Daxx and TCF4 interaction links to oral squamous cell carcinoma growth by promoting cell cycle progression via induction of cyclin D1 expression

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Protocol publication

[…] Total RNA was extracted from these cells using TRIzol (Life Technologies, Carlsbad, CA, USA) or Total RNA Miniprep Purification kit (GeneMark, Taipei, Taiwan). Five micrograms of RNA from each sample were then reverse transcribed using Superscript III Reverse Transcriptase (Life Technologies). RT-quantitative PCR (RT-qPCR) was performed using SYBR Green PCR master mix (Life Technologies) and an ABI 7500 sequence detection system (Life Technologies). The RT-qPCR primers used were as follows: Daxx forward, 5′-TGC AGA CAC CCC CGA AGC CT-3′; Daxx reverse primer, 5′-TGC CAT TCC ACT AGG GCC CTC A-3′; GAPDH forward, 5′-TCT TTT GCG TCG CCA GCC GAG-3′; GAPDH reverse primer, 5′-TGA CCA GGC GCC CAA TAC GAC-3′; cyclin D1 forward primer, 5′-TGT GAC CCG GAC TGC CTC CG-3′; and cyclin D1 reverse primer, 5′-GCG CAG GCT TGA CTC CAG CA-3′. [...] All analyses performed with SigmaPlot software. The two-tailed Students’s t test was used to evaluate the significance of the differences between two groups of data in all experiments. Values of P < 0.05 were considered significant. […]

Pipeline specifications

Software tools GeneMark, SigmaPlot
Applications Miscellaneous, Genome annotation
Organisms Homo sapiens
Diseases Carcinoma, Squamous Cell, Neoplasms, Ovarian Neoplasms, Prostatic Neoplasms