Computational protocol: The caudal regeneration blastema is an accumulation of rapidly proliferating stem cells in the flatworm Macrostomum lignano

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Protocol publication

[…] For live squeeze preparations, stained whole mounts and sections, a Reichert Jung Polyvar and a Leica DM 5000 light microscope were used. Photographs were taken with a Pixera Penguin 600CL and a The Imaging Source DFK 41F02 camera. Picture editing and drawing schemes was done with the free programs Inkscape [] up to version 0.45 and GIMP [] up to version 2.2.13. Some stained whole mounts were observed with confocal Zeiss LSM 510 and 710 microscopes. […]

Pipeline specifications

Software tools Inkscape, GIMP
Application Miscellaneous
Chemicals Bromodeoxyuridine