Dataset features

Specifications


Application: ChIP-on-chip analysis
Number of samples: 20
Release date: Dec 31 2007
Last update date: Mar 17 2012
Access: Public
Dataset link Meiotic DNA double strand breaks in the yeast Saccaromyces cerevisiae

Experimental Protocol


We use two different strategies to map the genome-wide distribution of meiotic DSBs in the yeast Saccharomyces cerevisiae. The first is a chromatin immunoprecipitation (ChIP) based approach that targets the Spo11p protein, which remains covalently attached to DSB ends in the rad50S mutant background. The second approach involves BND cellulose enrichment of the single strand DNA (ssDNA) recombination intermediate formed by end-resection at DSB sites following Spo11p removal. We use dmc1 and dmc1 rad51 mutants that accumulates meiotic single strand DNA intermediates

Repositories


GEO

GSE8981

ArrayExpress

E-GEOD-8981

BioProject

PRJNA102441

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Contact


Michael Lichten

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