Dataset features

Specifications


Dataset type: Other
Number of samples: 27
Release date: Dec 14 2017
Last update date: Nov 27 2018
Access: Public
Dataset link Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein-RNA crosslinking

Experimental Protocol


27 samples total, from five experimental courses (negative controls, and mitochondrial, nuclear, cytosolic, and endoplasmic reticulum-targeted APEX-RIP). Negative controls: 4 samples (2 replicates, with paired inputs and mock RIPs); Mitochondrial APEX-RIP: 6 samples (3 replicates, with paired inputs and RIPs); Nuclear APEX-RIP: 6 samples (3 replicates, with paired inputs and RIPs); Cytosolic APEX-RIP: 6 samples (6 replicates, with paired inputs and RIPs); ER-targeted APEX-RIP ("KDEL"): 4 samples (2 replicates, with paired inputs and RIPs).

Repositories


GEO

GSE106493

ENA

SRP123582

BioProject

PRJNA417050

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Contact


William Mallard