Computational protocol: Bacterial Cytological Profiling (BCP) as a Rapid and Accurate Antimicrobial Susceptibility Testing Method for Staphylococcus aureus

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Protocol publication

[…] The threshold for the deconvolved images was adjusted on FIJI (ImageJ 2.0.0) in order to remove background and obtain shape edges. All SYTOX and DAPI intensity measurements were performed on nondeconvolved images. Cell measurements were made using CellProfiler 2.0 (). Each parameter is taken as the weighted average of three images. For each strain, three sets of profiles (each represented by three images), were obtained in three experiments on different days (n = 3). […]

Pipeline specifications

Software tools ImageJ, CellProfiler
Application Microscopic phenotype analysis
Organisms Staphylococcus aureus, Epipremnum aureum
Diseases Bacterial Infections, Substance-Related Disorders
Chemicals Methicillin, Daptomycin, beta-Lactams