Dataset features

Specifications


Application: RNA-seq analysis, sRNA-seq analysis
Number of samples: 28
Release date: May 8 2015
Last update date: Oct 10 2018
Access: Public
Chemicals: Palmitic Acid
Dataset link A potential role for snoRNA in PKR activation during metabolic stress

Experimental Protocol


MEF cells reconstituted with wild type PKR (PKR_WT) or PKR with a mutation in each dsRBM (PKR_RM) were mock- or palmitate-treated. RNAs associated with PKR were immunoprecipitated. IPed RNAs were extracted, cloned and sequenced. We performed differential expression to obtain RNAs enriched in PKRWT after palmitate treatment. We sequenced three different datasets of samples (A, B and C). Each A and B dataset contains three biological replicates. Dataset C contains a single sample.

Repositories


GEO

GSE66540

ArrayExpress

E-GEOD-66540

ENA

SRP055866

BioProject

PRJNA277331

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