Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 23
Release date: Apr 27 2018
Last update date: Jul 30 2018
Access: Public
Chemicals: Bile Acids and Salts
Dataset link Transcriptomic Analysis of Hepatic Cells in Multicellular Organotypic Liver Models

Experimental Protocol


We measured gene expression in hepatocytes, LSECs, and Kupffer cells from the 3DHL and 3DHLK models at day 12 to better understand LSEC function in in vitro liver cultures. For additional comparisons, we collected gene expression from hepatocytes in a collagen sandwich (CS) and hepatocyte monolayer (HM) as well as Kupffer cells at day 3 prior to their inclusion in the 3D liver model. We obtained Affymetrix microarray data for 23 samples, considering three cell-types and across four culture systems. Each cell-type culture system was performed in triplicate, except Kupffer cells at day 12 was performed in duplicate.

Repositories


GEO

GSE74424

BioProject

PRJNA300659

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Contact


T. M. Murali