|Application:||Gene expression microarray analysis|
|Number of samples:||18|
|Release date:||Dec 15 2013|
|Last update date:||Aug 10 2018|
|Diseases:||Disorders of Sex Development|
|Dataset link||Molecular Mapping of Keratinocyte Differentiation by Functional Gene Expression Profiling|
Primary human keratinocytes from three donors were subjected to CID in separate cultures and harvested at successive time points including 2 days prior to reaching confluence (D(-2)), at the point of confluence (D(0)), as well as after reaching confluence (D(1), D(2) and D(3)). In order to minimize any donor and sample effects, the RNA samples were pooled at each time point and gene expression profiling was performed (Affymetrix U133 A & B). Secondary validation study was performed with three additional time observation arrayed in Affymetrix U133A2 platform for the 3 patient pool. The time observations were: D(-3),D(-2),D(-1),D(0),D(+1),D(+2),D(+3),D(+5).