Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Dec 15 2013
Last update date: Aug 10 2018
Access: Public
Diseases: Disorders of Sex Development
Dataset link Molecular Mapping of Keratinocyte Differentiation by Functional Gene Expression Profiling

Experimental Protocol


Primary human keratinocytes from three donors were subjected to CID in separate cultures and harvested at successive time points including 2 days prior to reaching confluence (D(-2)), at the point of confluence (D(0)), as well as after reaching confluence (D(1), D(2) and D(3)). In order to minimize any donor and sample effects, the RNA samples were pooled at each time point and gene expression profiling was performed (Affymetrix U133 A & B). Secondary validation study was performed with three additional time observation arrayed in Affymetrix U133A2 platform for the 3 patient pool. The time observations were: D(-3),D(-2),D(-1),D(0),D(+1),D(+2),D(+3),D(+5).

Repositories


GEO

GSE26688

ArrayExpress

E-GEOD-26688

BioProject

PRJNA136595

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Contact


Yoga Balagurunathan