Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Nov 30 2008
Last update date: Oct 29 2018
Access: Public
Diseases: Tumor Lysis Syndrome
Dataset link Zalypsis: A novel marine-derived compound with potent antimyeloma activity

Experimental Protocol


MM cells treated in vitro with Zalypsis (5 nM) were harvested at the beginning of induction of cell death (15-20% cell death as assessed by Annexin V-FITC staining). Total RNA was extracted using Trizol reagent (Life Technologies, MD, USA) and purified with RNAeasy Mini Kit (Qiagen, CA, USA). RNA integrity was verified with the Agilent 2100 Bioanalyzer (Agilent, CA, USA). Double-stranded cDNA and biotinylated cRNA were synthesized with T7-polyT primer and the BioArray RNA labeling kit (Enzo, NY, USA), respectively. The labelled RNA was then fragmented and hybridized to HG-U133 Plus 2.0 oligonucleotide arrays (Affymetrix, CA, USA), which were scanned in a Gene Array Scanner and analyzed using the DNA-Chip Analyzer software (DChip). Changes in gene expression greater than two-fold were considered significant.

Repositories


GEO

GSE13662

ArrayExpress

E-GEOD-13662

BioProject

PRJNA110213

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Contact


Enrique Ocio
Enrique M. Ocio