Computational protocol: Siglec-7 expression is reduced on a natural killer (NK) cell subset of obese humans

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Protocol publication

[…] Flow cytometry was performed using a LSR Fortessa with BD FACSDiva Flow Cytometry Software Version 6.2 (BD Biosciences). Compensation was done with BD™ CompBeads Set Anti-Mouse Ig, κ (BD Biosciences). For gating the Siglec positive cells, a tube without Siglec antibodies (fluorescence minus one (FMO)) served as control. Furthermore, an isotype control was used to visualize possible unspecific binding of the antibodies to FC receptors.Data was analysed using FACSDiva Flow Cytometry Software Version 6.2 and FlowJO 10 (FlowJo LLC, Ashland, USA). […]

Pipeline specifications

Software tools BD FACSDiva, FlowJo
Application Flow cytometry
Organisms Homo sapiens, Human poliovirus 1 Mahoney
Diseases Neoplasms
Chemicals Sialic Acids, N-Acetylneuraminic Acid