Computational protocol: Methods to Evaluate Cell Growth, Viability, and Response to Treatment in a Tissue Engineered Breast Cancer Model

Similar protocols

Protocol publication

[…] Cell density, defined as the number of nucleated cells per 1 × 106 pixels (area), was determined from photomicrographs (400x) of H&E-stained histologic sections of surrogates (complete cross sections of each surrogate analyzed). The number of nucleated cells was either counted manually or determined using CellProfiler analysis–, as previously published. The number of nucleated cells was normalized to the cross-sectional area of each surrogate, measured using the polygon tool in ImageJ. […]

Pipeline specifications

Software tools CellProfiler, ImageJ
Applications Laser scanning microscopy, Microscopic phenotype analysis
Organisms Homo sapiens
Diseases Breast Neoplasms, Neoplasms
Chemicals Paclitaxel, Lactic Acid