Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Mar 10 2011
Last update date: Aug 23 2018
Access: Public
Chemicals: Oxygen
Genes: ITGAM
Dataset link Expression data from granulocytic MDSC (G-MDSC) and neutrophils

Experimental Protocol


G-MDSC (CD11b+Ly6G+Ly6Clow) were purifed from splenocytes in EL4 lymphoma tumor bearing mice by positive selection of Ly6G using microbeads isolation. Neutrophils were purified from ascitic fluids induced after injection of milk protein, casein by negative selection of F4/80 and positive selection of Ly6G using microbeads isolation. Their RNA was extracted and gene expression was analyzed using Affymetrix microarray.

Repositories


GEO

GSE24102

ArrayExpress

E-GEOD-24102

BioProject

PRJNA130307

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