Dataset features

Specifications


Application: sRNA-seq analysis
Number of samples: 4
Release date: Jul 13 2017
Last update date: Jul 13 2017
Access: Public
Dataset link Deep sequencing of uncapped RNA in rdr1/2/6 and wild-type Arabidopsis plants

Experimental Protocol


Untreated and 2hr drought stress-treated 2 weeks-old rdr1/2/6 and wild-type Arabidopsis thaliana plants were used. To determine 5’-end of uncapped RNA with and without poly (A), we developed Deep Sequencing of Uncapped RNAs (DSUR) with reference to PARE analysis (German et al. 2009, Nature Protocol 4:356-362) and applied DSUR using SOLiD to identify genome-wide RDR1/2/6-mediated antisense RNA loci in Arabidopsis.

Repositories


GEO

GSE39033

ENA

SRP013992

BioProject

PRJNA169644

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Contact


Motoaki Seki