Computational protocol: New taxonomic and conservation status of Ossiculum (Vandeae, Orchidaceae), a highly threatened and narrow-endemic angraecoid orchid from Central Africa

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Protocol publication

[…] On-site ecology and conservation status of Ossiculum aurantiacum were investigated during three main field campaigns in the Southwest Region of Cameroon (2004, 2011 and 2017). To look for additional collections, herbarium specimens of BR, BRLU, K, WAG and YA (herbaria acronyms according to ) were examined. The geographical distribution of the species was determined from data given on the herbarium sheets. New country records were identified by comparing the species distribution with the information provided by . The species distribution map was prepared using ArcMap 10.4.1 ().Phylogenetic relationships between African angraecoid orchids, including Ossiculum and Calyptrochilum, were inferred on the basis of DNA sequence data from three regions (ITS, matK, and the trnL-trnF intergenic spacer) and 555 accessions representing 316 species from 43 genera (see ). We performed the parsimony and the Bayesian analyses on the combined matrix using, respectively, the computer programme PAUP* v.4.0 a 146 () and MrBayes 3.2.5 (; ).Using the IUCN Red List Categories and Criteria (), we made a preliminary risk of extinction assessment for the species. We imported georeferenced specimen data into the ConR package () to calculate the area of occupancy (AOO) and extent of occurrence (EOO). The cell size for AOO was set 2 × 2 km as recommended by . We calculated the number of ‘locations’ (as defined by ) with regard to the kind of threats, such that a single ‘location’ may encompass more than one adjacent population. […]

Pipeline specifications

Software tools PAUP*, MrBayes
Application Phylogenetics