Computational protocol: Anti-ganglioside antibodies are removed from circulation in mice by neuronal endocytosis

Similar protocols

Protocol publication

[…] Images for analysis of fluorescent intensity were captured using an LSM 5 Pascal confocal microscope and image analysis was performed using ImageJ software as previously described (). Illustrative images were captured using a Zeiss AxioImager Z1 microscope with ApoTome attachment. For antibody intensity measurements in triangularis sterni preparations, 15 images were taken per triangularis sterni. For spinal cord, nine images were captured per mouse. [...] Mice were assigned to each experiment depending on the availability of age-matched mice across genotypes. Mice were coded and assigned to genotype groups after analysis. Sample size estimation was chosen using G*power software (v 3.1.9.2), with an α-error of 0.05 and a power of 0.8. For in vivo clearance studies, based on preliminary data, an effect size of 2.5 was chosen, while for antibody intensity measurements (where individual NMJs or NeuN positive cells were ranked) an effect size of 0.4 was chosen. Sample sizes also corresponded with previous similar studies (). […]

Pipeline specifications

Software tools ImageJ, G*Power
Applications Miscellaneous, Laser scanning microscopy, Microscopic phenotype analysis
Organisms Mus musculus
Diseases Guillain-Barre Syndrome