Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 40
Release date: Feb 14 2010
Last update date: Mar 20 2012
Access: Public
Diseases: Metabolic Diseases, Neoplasms, Adipose Tissue
Dataset link A saturated fatty acid rich diet can induce an 'obese-like' gene expression profile in adipose tissue

Experimental Protocol


In a parallel controlled feeding trial, subcutaneous adipose tissue samples were collected from moderately overweight human subjects at baseline and after 8 weeks consumption of either SFA-rich or MUFA-rich diets. RNA was isolated from the samples using Trizol and the Qiagen RNeasy Micro kit (Qiagen, Venlo, the Netherlands). RNA was was labeled using a one-cycle cDNA labeling kit (MessageAmpTM II-Biotin Enhanced Kit, Ambion, Inc.) and hybridized to Affymetrix NuGO_Hs1a52018 arrays.

Repositories


GEO

GSE14954

ArrayExpress

E-GEOD-14954

BioProject

PRJNA111821

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Contact


Guido Hooiveld