|Application:||Gene expression microarray analysis|
|Number of samples:||40|
|Release date:||Feb 14 2010|
|Last update date:||Mar 20 2012|
|Diseases:||Metabolic Diseases, Neoplasms, Adipose Tissue|
|Dataset link||A saturated fatty acid rich diet can induce an 'obese-like' gene expression profile in adipose tissue|
In a parallel controlled feeding trial, subcutaneous adipose tissue samples were collected from moderately overweight human subjects at baseline and after 8 weeks consumption of either SFA-rich or MUFA-rich diets. RNA was isolated from the samples using Trizol and the Qiagen RNeasy Micro kit (Qiagen, Venlo, the Netherlands). RNA was was labeled using a one-cycle cDNA labeling kit (MessageAmpTM II-Biotin Enhanced Kit, Ambion, Inc.) and hybridized to Affymetrix NuGO_Hs1a52018 arrays.