Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 9
Release date: Oct 23 2009
Last update date: Aug 23 2018
Access: Public
Dataset link WNT4 is required for ovarian follicle development and female fertility

Experimental Protocol


Granulosa cells were obtained from 20-26 day-old mice of various genotypes 48h after eCG treatment, using the needle puncture method as previously described. Cells were then infected with adenoviruses to express eGFP or Cre, or overexpress WNT4 in serum-free medium, and subsequently harvested for RNA extraction as described below. Preliminary experiments demonstrated that an infection efficiency of >80% could be obtained at an MOI of ~50 (as determined by analysis of fluorescent signal in Ad-eGFP-infected cells) and that the Ad-Cre and Ad-WNT4 viruses produced efficient recombination of the floxed Ctnnb1 alleles and robust WNT4 overexpression, respectively. Microarray analyses were done using triplicate RNA samples from each adenoviral treatment described above, and using mouse expression set 430 microarrays (Affymetrix, Santa Clara, CA).

Repositories


GEO

GSE18704

ArrayExpress

E-GEOD-18704

BioProject

PRJNA121495

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Contact


Derek Boerboom