Dataset features

Specifications


Application: ChIP-on-chip analysis
Number of samples: 16
Release date: May 20 2009
Last update date: Mar 21 2012
Access: Public
Chemicals: Cocaine
Dataset link Genome-Wide Promoter Analysis of Epigenetic Regulation by Cocaine (MM8 data)

Experimental Protocol


Mice were treated with cocaine or saline. Chromatin immunoprecipitation was performed for methylated histone H3 lysine 9 and 27, ∆FosB, and phospho-CREB as described previously (Kumar et al., 2005) with minor modifications. Immunoprecipitated DNA was amplified via ligation-mediated PCR and hybridized to Nimblgen mouse MM8 promoter arrays. 2-3 biological replicates per condition.

Repositories


GEO

GSE16184

ArrayExpress

E-GEOD-16184

BioProject

PRJNA122833

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Contact


guanghua xiao