Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 48
Release date: Jun 3 2011
Last update date: Mar 23 2012
Access: Public
Chemicals: Iron
Computational protocol: SBEAMS, TM4, Gaggle, BioProspector, MEME, WebLogo
Dataset link Two transcription factors are necessary for iron homeostasis in a salt-dwelling archaeon [gene expression data]

Experimental Protocol


The Δura3 parent, Δidr2 and Δidr1, and Δ idr1Δidr2 mutant strains were grown to mid-logarithmic phase (OD600 ~0.4 – 0.8) in CDM with all trace metals except iron. Cultures were split in half and FeSO4 was added to one half, while the other was continued under iron limitation. 8-mL samples were collected from each culture every 20 minutes for 60 minutes (see also experimental design, Supplementary Figure 1, Schmid et al., 2011). RNA from two biological replicate time courses were prepared, averages of these replicates are reported in the published study, whereas data from each replicate are reported here. The zero time point was harvested immediately before the addition of iron. Each Sample is based on two arrrays (one with dye-swap).

Repositories


GEO

GSE29704

ArrayExpress

E-GEOD-29704

BioProject

PRJNA153853

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Contact


Amy Schmid
Amy K Schmid

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