Dataset features
Specifications
Application: | Gene expression microarray analysis, ChIP-on-chip analysis |
---|---|
Number of samples: | 5 |
Release date: | Mar 14 2013 |
Last update date: | May 27 2014 |
Access: | Public |
Genes: | CENPA, RAD51 |
Taxon: | Schizosaccharomyces pombe |
Dataset link | DNA topoisomerase III localizes to centromeres and affects centromeric CENP-A levels in fission yeast |
Experimental Protocol
For transcription: Total RNA from top3-105 mutant and WT control cells after 8 hours at 36C in biological duplicates.
For Top3-myc chromatin immunoprecipitation: DNA immunoprecipitated with mouse anti-Myc using chromatin extracts from cells expressing Top3-Myc from the endogenous locus at 30C in biological duplicates normalized to input DNA from wild type cells at 30C in biological duplicates.
For CENP-A/Cnp1 chromatin immunoprecipitation: DNA immunoprecipitated with anti-Cnp1 serum using chromatin extracts from top3-105 mutant and wild type control cells after 8 hours at 36C in in biological duplicates normalized to input DNA from each strain.
Repositories
Download
Contact
Karl Ekwall
Dataset Statistics
info
Citations per year
Dataset publication
2013
PLoS Genet
PMCID: 3597498
PMID: 23516381
DOI: 10.1371/journal.pgen.1003371