Dataset features

Specifications


Application: Gene expression microarray analysis, ChIP-on-chip analysis
Number of samples: 5
Release date: Mar 14 2013
Last update date: May 27 2014
Access: Public
Genes: CENPA, RAD51
Taxon: Schizosaccharomyces pombe
Dataset link DNA topoisomerase III localizes to centromeres and affects centromeric CENP-A levels in fission yeast

Experimental Protocol


For transcription: Total RNA from top3-105 mutant and WT control cells after 8 hours at 36C in biological duplicates. For Top3-myc chromatin immunoprecipitation: DNA immunoprecipitated with mouse anti-Myc using chromatin extracts from cells expressing Top3-Myc from the endogenous locus at 30C in biological duplicates normalized to input DNA from wild type cells at 30C in biological duplicates. For CENP-A/Cnp1 chromatin immunoprecipitation: DNA immunoprecipitated with anti-Cnp1 serum using chromatin extracts from top3-105 mutant and wild type control cells after 8 hours at 36C in in biological duplicates normalized to input DNA from each strain.

Repositories


GEO

GSE44206

ArrayExpress

E-GEOD-44206

BioProject

PRJNA189154

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Contact


Karl Ekwall

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