Dataset features


Application: Gene expression microarray analysis
Number of samples: 27
Release date: Jun 30 2017
Last update date: Jul 25 2018
Access: Public
Taxon: Mus musculus
Dataset link Testis transcriptional profiling of males deriving from two high fertility mouse lines (FL1, FL2) which have been long-term selected for the characteristics large and heavy litters in comparison to randomly selected control line (Ctrl)

Experimental Protocol

Two separated microarray experiments (sets) were performed. Initially, for array set I each testis transcriptome of 8 mouse bucks per line was hybridized to 8 single microarray chips (n=8). These males are deriving from the 4th of entirely 5 replicates of a recently accomplished comprehensive two-factorial breeding experiment with the aim to scrutinize the impact of males and females on fertility parameters (recently submitted (08.16)). Beside the accompanying effect of being able to correlate breeding experiment observations with gene expression results, along the first array set we had the objective to develop a first profound hint about differential gene expression between these three lines. The resulting lists were experimentally verified with a second microarray set which was based on testis samples from 8 biologically independent bucks per line also involved in the two-factorial breeding experiment, 2nd replicate (n=8). Contrary to the first array set, a testis-RNA pool of the separated line specimens had been generated before these line pools were hybridized to one microarray regarded as pool-chip. By means of this approach we ensured a profound verification of the entirety of gained gene expression results. This handily served for exclusion of ‘false positive’ transcripts which were initially indicated as differentially expressed but then could not keep validity in biologically independent reevaluation.








Alexander Sobczak