Computational protocol: Langerhans cell (LC) proliferation mediates neonatal development, homeostasis, and inflammation associated expansion of the epidermal LC network

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Protocol publication

[…] All Z-stack acquisitions were done using a confocal microscope (SP5; Leica) with 20× and 40× lenses. The number of LCs per square millimeter of epidermis was determined by acquiring Z-stacks of immunolabeled epidermal sheets. A minimum of four Z-stacks (0.56 mm2/Z-stack) were acquired per epidermal sheet. The number of cells per Z-stack was enumerated and represented as cells per square millimeter. The distance between a langerin-GFP+ cell and its closest GFP+ neighbor (Ki67+ or Ki67−) was measured on a maximal intensity projection of Z-stacks of immunolabeled epidermal sheets. All image quantification and rendering was performed on MetaMorph (MDS Analytical Technologies) and Imaris (Bitplane) software. […]

Pipeline specifications

Software tools MetaMorph, Imaris
Organisms Mus musculus, Homo sapiens
Diseases Alzheimer Disease, Dermatitis, Atopic, Inflammation