|Number of samples:||19|
|Release date:||Feb 5 2018|
|Last update date:||Nov 27 2018|
|Dataset link||Crosstalk between the RNA methylation and histone binding activities of MePCE regulates P-TEFb activation on chromatin|
RNA-Seq was performed in MDA-MB-231 shNC (Negative Control) and shMePCE cells. Concomitantly, ChIP-seq was performed in these same cells with antibodies specifically recognizing RNA Polymerase II phosphorylated at S2 or S5 of its CTD, the N-ter of MePCE, as well as GFP (Negative Control) and H3K4me3 (Positive Control). Because, the ChIP-Seq with the MePCE antibody does not work well for single gene inspection, we performed additional ChIP-seq with the MePCE antibody in HeLa-S3-FlpIn cells.