Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 19
Release date: Feb 5 2018
Last update date: Nov 27 2018
Access: Public
Dataset link Crosstalk between the RNA methylation and histone binding activities of MePCE regulates P-TEFb activation on chromatin

Experimental Protocol


RNA-Seq was performed in MDA-MB-231 shNC (Negative Control) and shMePCE cells. Concomitantly, ChIP-seq was performed in these same cells with antibodies specifically recognizing RNA Polymerase II phosphorylated at S2 or S5 of its CTD, the N-ter of MePCE, as well as GFP (Negative Control) and H3K4me3 (Positive Control). Because, the ChIP-Seq with the MePCE antibody does not work well for single gene inspection, we performed additional ChIP-seq with the MePCE antibody in HeLa-S3-FlpIn cells.

Repositories


GEO

GSE108830

ENA

SRP128133

BioProject

PRJNA428755

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