Computational protocol: Comparative Analysis of Carbon Monoxide Tolerance among Thermoanaerobacter Species

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Protocol publication

[…] PCR products obtained from 16S rRNA gene amplification were purified using the PCR Clean Up kit NucleoSpin Extract II (Macherey-Nagel, Düren, Germany) and sequenced directly at Eurofins MWG Operon (Ebersberg, Germany). Partial sequences were assembled using the alignment editor BioEdit v7.0.9 software package (Hall, ). Similarity searches for the 16S rRNA gene sequence derived from strain PCO were performed using the NCBI BLAST search program within the GenBank database (Altschul et al., ). The 16S rRNA gene sequence of Thermoanaerobacter strain PCO is available in the DDBL/EMBL/GenBank databases under the accession number HF586422. […]

Pipeline specifications

Software tools BioEdit, BLASTN
Application Phylogenetics
Organisms Thermoanaerobacter pseudethanolicus, Thermoanaerobacter brockii, Thermoanaerobacter wiegelii
Chemicals Ethanol, Carbon Monoxide, Glucose, Hydrogen, Lactic Acid