Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 14
Release date: Aug 24 2017
Last update date: Jan 24 2018
Access: Public
Diseases: Starvation
Chemicals: Carbon, Methane, Lactic Acid, Succinic Acid
Computational protocol: BWA, SAMtools, HTSeq, DESeq2
Dataset link OXYGEN-LIMITED METABOLISM IN THE METHANOTROPH METHYLOMICROBIUM BURYATENSE 5GB1C

Experimental Protocol


RNA-seq analysis of gene expression of M. buryatense 5GB1 grown in continuous bioreactor culture. Several conditions were used to isolate the effects of O2 limitation on 5GB1 metabolism: O2 limitation at slow growth, O2 limitation with fast growth, CH4 limitation with fast growth, and a terminal oxidase knockout mutant. Each condition had two biological replicates, and between 1 and 4 technical replicates.

Repositories


GEO

GSE101981

ENA

SRP113731

BioProject

PRJNA396065

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Contact


Mitchell Pesesky

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