Computational protocol: Correlation between Mitochondrial Reactive Oxygen and Severity of Atherosclerosis

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Protocol publication

[…] In situ perfused hearts were excised and embedded in Tissue-Tek OCT compound (Sakura, USA), frozen at −80°C, cut in 10 μm sections along 480 μm aorta length from the aortic valve leaflets, and stained by Oil Red O according to Paigen et al. []. The lipid-stained lesions were quantified as described by Rubin et al. [] using the ImageJ (1.45 h) software. [...] The results are presented as the means ± SE. The comparisons between the groups were analyzed by one-way ANOVA with posttest of Tukey. For correlation analyses, we performed Spearman's univariate correlation test, partial (adjusted) correlation, and multiple linear regressions models, using, respectively, GraphPad Prism 5.0, SPSS Statistics 14.0, and R package 2.9. In the multiple linear regressions analyses, variable selection in each model was performed using the regsubsets tool available in the leaps package for R [, ], which identifies the exploratory variables that create the best-fitting linear regression models according to Bayesian Information Criterion (BIC). The level of significance was set at P < 0.05. […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Mus musculus
Diseases Liver Diseases, Atherosclerosis
Chemicals Cholesterol, NADP, Oxygen